Category Archives: carbamate

carbamate, Chromatography, Chromatography Quiz

Chromatography Quiz #33

September 26, 2019 Pickering Laboratories

Chromatography Quiz #32: I’m not seeing any peaks! Carbamates edition – RESULTS

Pickering Labs would like to congratulate all of our winners for our previous newsletter’s I’m not seeing any peaks! Carbamates edition Quiz: Jim Balk from Nebraska Public Health Environmental Lab, Josiah Hakala from Minnesota Department of Health, Narjes Ghafoori from LA County Environmental Toxicology Lab, Tom Schneider from Suffolk County Water Authority, and Jiufeng Fan from Glaxo Smith Kline.

Winners will soon receive: A Tile Pro Combo from Amazon.com! Tile is a tiny Bluetooth tracker and easy-to-use app that helps you find everyday items in seconds. Sleek, durable and water-resistance, the Tile Pro seamlessly pairs with your smartphone. The easiest way to find your things!

Congratulations to our quiz winners!

Thank you all for your submissions!

The correct answers for the “I’m not seeing any peaks!” Carbamates edition quiz:

The reactor temperature was set too low. In normal operation, the reactor temperature should be set to 100 °C for proper post-column reaction completion. The incomplete reaction will give you low response for all analytes except Carbaryl and 1-Naphthol. Carbaryl becomes 1-Naphthol after the first step in the post-column reaction and 1-Naphthol is naturally fluorescent and does not require the OPA reaction.

Chromatography Quiz 33 – What the Brij?!?

Correctly answer the question below and win a prize! Simply email your answer and your full contact information to Rebecca at rlsmith@pickeringlabs.com by November 1, 2019 in order to win. You will receive email confirmation when your submission is received, and the troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).

Below you will find the method details for the Fumonisins method. What is the purpose of adding 30% Brij to the post-column derivatizing reagent?

(Full method abstract(s) available on our website.)

carbamate, Chromatography Quiz, glyphosate

Chromatography Quiz No.14

October 14, 2013 homerjs9

Chromatography Quiz #13 Results

We would like to congratulate our grand prize winners of our last newsletter’s Carbamate Analysis Chromatography Quiz: Irene Taylor from Orange County Utilities, Jim Balk from DHHS Public Health Environmental Laboratory, Narjes Ghafoori from LA County Environmental Toxicology Laboratory, Helene Lachance from Shur-Gain Nutreco, and Jamie Palmer and Matthew Hartz from Underwriters Laboratories!!!

They have each won and will be receiving: a Laser Board Game from Sharper Image! (This strategy-based board game is similar to chess and features an 80-square Egyptian themed grid. The goal is to protect your game pieces while eliminating your opponent’s by bouncing eye-safe lasers at them through the maze of mirrors you’ve constructed.)

We would like to thank all of you for your submissions!

The correct answer for the modified Carbamate chromatogram: Reagent #2 (CB910, OPA, and Thiofluor) was partially oxidized. The peak heights of Carbaryl and 1-Naphthol have significant diagnostic importance in regard to the OPA reagent system. Since 1-Naphthol naturally fluoresces, we can eliminate the fluorescence detector as the problem. Though smaller peak sizes can reflect problems with Reagent #1, since the Carbaryl peak is full-sized, we can deduce that hydrolysis has occurred (Carbaryl naturally fluoresces after hydrolysis).

Particularly astute observation yields additional details about our troubleshooting chromatogram – slightly shifting retention times towards the end of the run could indicate a problem with HPLC hardware, such as a proportioning valve.

Thank you!

Pickering Labs

Chromatography Quiz #14:

Complete the cross word puzzle below and win a prize! Simply email your answer (copy of completed puzzle or list answers with clues) as well as your full contact information to Rebecca at rlsmith@pickeringlabs.com by December 15, 2013 in order to win. You will receive email confirmation that your submission has been received. The answer to the puzzle and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).

Carbamate/Glyphosate Analysis: Cross Word Puzzle

carbamate, clean-up, eluvac, freestyle, glyphosate, GPC, NEMC, pinnacle pcx, post-column derivatization, Uncategorized

NEMC 2013

August 13, 2013 homerjs9

Pickering Laboratories participated in the National Environmental Monitoring Conference in San Antonio, Texas from August 4-8.

Rebecca and Wendy were on-hand to share Pickering’s products with the environmental laboratories involved with the conference. On display were the new FREESTYLE sample clean-up system and the Pinnacle PCX.

Some highlights of the conference included a new product by InnovaPrep, which will rapidly concentrate pathogens in liquid samples, and the 2-D GCMS analysis of POPs in human milk. There were also several interesting discussions about pesticide extraction and analysis as well as the use of Hydrogen as a carrier gas in GC (instead of Helium, for which there is currently a shortage).

The meeting was held at the Hyatt in downtown San Antonio on the Riverwalk. This area of town is full of history and interesting architecture. We were also located directly across the plaza from the Alamo.

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Pickering Laboratories in 2013

June 25, 2013 homerjs9 1 Comment

By Wendy Rasmussen

In recent years, we have released several new products and applications, and with still more on the horizon, it occurred to me that now would be a great time to summarize the Pickering of today – our mindset and our wide variety of products & applications.

No longer are we simply the “Post-Column Company”. We are the “Automated-Sample Antioxidants” company. Think of us as the new “super fruit.” The Acai berry, or perhaps the new Chia Seeds (incidentally, we do have a post-column application for the identification & quantitation of Antioxidants in a variety of matrices).

We are still very active, and we as a company plan to be here for many years to come. We are still the company founded on chemistry and a desire to to teach, to spread our technical expertise, to support our customers.

It’s been a few years now since Pickering began distributing and supporting our LCTech Product line. The products have shown an ever increasing interest here in the US and in Canada (our official Sales Territory for this product line). We are very proud to offer these products and we hope we can develop it further in the future.

Historically, we have provided the back-end of an analysis (post-column derivatization). Nowadays, we can provide the front-end of analysis as well (the sample-cleanup).

In thinking about our product offerings, I realized that a simple list does not effectively show the scope of the products we have to offer in 2013 – primarily because we have a lot of overlap between products and product lines. We’re not a vertical company in that regard. I suppose one could say our product offerings are more circular in that many do not fall into a single distinct category. I am a very visual person, and for me, a Venn diagram and our overall “product scale” really helped to understand and clarify our products:

General "Product Scale" for Pickering Offerings — General “Product Scale” for Pickering Offerings

For those of who like lists, you can find one Here, on Pickering’s website, and on LCTech’s Website

For any Questions, please feel free to contact us:

Pickering Laboratories, Inc.
Mountain View, California
Email: sales@pickeringlabs.com
Phone: (direct) 650-694-6700 or (toll-free) 800-654-3330

Image of Acai Berries: http://acaiberryeducation.com/

carbamate, Chromatography Quiz, pickering laboratories, post-column derivatization

Chromatography Quiz No. 13

June 24, 2013 homerjs9

Chromatography Quiz #12 Results

We would like to congratulate our grand prize winners of our last newsletter’s Amino Acid Analysis Chromatography Quiz: Luiz Paulo Mousinho from Chemetric in Brazil, Helene Lachance from Shur-Gain Nutreco in Canada, and Narjes Ghafoori from the LA County Environmental Toxicology Laboratory!!!

They have each won and will be receiving: a $100 gift card from Amazon.com! We would like to thank all of you for your submissions.

The correct answer for the modified Amino Acids chromatogram: the back pressure regulator on the outlet of the detector stopped functioning properly. This was indeed another hardware troubleshooting quiz! The pattern of noise seen in the customer’s baseline is characteristic to this problem, and a new back pressure regulator eliminated the noise.

Thank you!
Pickering Labs

Chromatography Quiz #13:
Identify the error made when running the Carbamate chromatogram below and win a prize! Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.com by August 30, 2013 in order to win. You will receive email confirmation that your submission has been received. The troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission).

Carbamate Analysis for US EPA Method 531.1

Pickering Standard: 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 10 µL injection

Pickering Column: 1846250 Carbamate Column, C₁₈, 4.6 x 250 mm

Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)

Column Temperature: 42 °C

Flow rate: 1 mL/min

Eluent Gradient:

TIME	WATER	MeOH %
0	85	15
1	85	15
44	25	75
44.1	0	100
49	0	100
49.1	85	15
57	85	15

Post-column conditions:

Reagent 1: Hydrolysis reagent CB130

Reagent 2: 100 mg of OPA, 2 g Thiofluor™ in 950 mL of CB910

Reactor 1: 100 °C, 0.5 mL

Reactor 2: ambient. 0.1 mL

Reagent flow rates: 0.3 mL/min

Detection: Fluorometer ex 330 nm, em 465 nm

Troubleshooting Chromatogram:

Reference Chromatogram:

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Pickering Exhibits at Pittcon 2013

June 12, 2013 homerjs9

This year’s Pittcon was held in Philadelphia, Pennsylvania from March 17-21, 2013.

On display were Pinnacle PCX, EluVAC, and a video of the FREESTYLE Automated Sample Preparation Instrument. The video was a new technique for us, and it was well received.

The video in our booth was a combination of the separate videos for each module of the FREESTYLE Automated Sample Preparation Instrument. You can view the originals here (they are very informative, and we highly recommend them): FREESTYLE VIDEOS

Wendy, Mike and David enjoyed visiting with existing and future customers, as well as our distributors from around the world, including our Canadian distributor, Chromatographic Specialties.

Before the show opened, we had the opportunity to explore the city. Philadelphia is one of our oldest cities and is full of history and landmarks. Some of this editor’s favorites include Independence Hall and the surrounding neighborhoods, and the Art Museum (see more below). On this trip, we explored Reading Terminal Market, and sampled that famous cuisine, the Philly Cheese steak Sandwich. I’m told it is not truly a Philly Steak sandwich unless you have it with Cheez Whiz. Any type of real cheese doesn’t count. I made that mistake on my first trip to Philadelphia, and the man behind the counter let me know it! I wasn’t about to make that mistake again:

The Philadelphia Museum of Art is known for its amazing collection of artwork (viewed on a previous visit), but is also made famous by the scene of Sylvester Stallone running up the stairs in the movie Rocky. Since the author of this entry happened to be training for a race, she decided that a little cold wasn’t going to stop her. So she put on her iPod (Rocky Theme playing, of course), and ran from the hotel to the top of the steps and back (total distance: about 2.8miles).

On the return to the hotel, I came across a version of the LOVE sculpture, by American Artist Robert Indiana. I always thought it was was much bigger…

For those of you interested in learning more about the sculpture, click HERE

If you’re ever in Philadelphia, the Philadelphia Museum of Art is definitely worth a visit.

But if you can’t make the trip to Philadelphia, you could also stay home and watch Rocky…

carbamate, glyphosate, laprw

Pickering Attends 4th Annual Latin American Pesticide Residue Workshop

June 10, 2013 homerjs9

By David Mazawa

The Fourth Latin American Pesticide Residue Workshop (LAPRW) was held from May 26 to 29, 2013 at the Compensar Convention Center in Bogotá, Colombia. About 400 people from universities, private companies, and government agencies responsible for monitoring and regulating pesticide residues in agricultural and environmental samples attended the valuable presentations, discussions, and workshops. About 19 different exhibitors, including Pickering Laboratories, proudly sponsored the 4^th LAPRW. We would like to thank the Universidad Nacional de Colombia, Sociedad Colombiana de Ciencias Químicas and the Scientific Committee of the Latin American Pesticide Residue Workshop for putting together a great show. We look forward to the advancements of the Fifth Latin American Pesticide Residue Workshop in Santiago, Chile.