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TRIONE® Ninhydrin Reagent

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Two bottles of Trione -- different sizesTRIONE® Ninhydrin Reagent

Chromatographic Grade™
A prepared Reagent for Automated Post-column Derivatization of Primary and Secondary Amines

TRIONE® Ninhydrin reagent is specially formulated for amino acid analysis. It contains Ninhydrin, Hydrindantin (reduced Ninhydrin), a Lithium Acetate buffer, and Sulfolane, a water-miscible organic solvent. The solvent is necessary to maintain the solubility of both the Hydrindantin and the primary amine product, Ruhemann’s Purple. The buffer is required because the reaction is pH dependent. The active ingredients—Ninhydrin and Hydrindantin are required for proper development of secondary and primary amines, respectively.

TRIONE® is so stable that is does not require refrigeration, either in shipment, storage, or in the reservoir. Quantitation is consistent from the first to the last mL, so there is no waste. The high signal-to-noise ratio of TRIONE®, when compared to DMSO-containing reagents, permits detection sensitivity to be increased with minimum increase in background noise — a feature particularly appreciated at sample concentrations of <50 picomoles.

Two Preparations are available to suit your usage and storage requirements:

T100 AND T100C

  • Pour into your reservoir and use; the ultimate in convenience with a minimum of handling
  • 4-month* shelf life


  • Combine two solutions, swirl, and use
  • 12-month* shelf life before mixing preparation; one month in the reservoir


* From date of manufacture



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Pricing & Ordering

[zentable type=eluants title=’TRIONE® NINHYDRIN REAGENT CATALOG INFORMATION’]T100 T100C T200[/zentable]
* From date of manufacture