All posts by homerjs9

On-Demand Webinar: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices

If you missed the live webinar last summer, you can still hear the Webinar On-Demand! Just click on the links below to launch the presentation.
On August 14th, 2012, in collaboration with Thermo Scientific, Pickering Laboratories participated in a free webinar titled: Rugged and Sensitive Analysis of Mycotoxins in Food Matrices
 
The webinar was hosted by LC/GC Magazine and Thermo Scientific.
 
Live Webcast: Tuesday, August, 14, 2012 at 8:00 am PDT; 11:00 am EDT; 15:00 GMT

Register Free at http://www.chromatographyonline.com/foodmatrices
EVENT OVERVIEW
Mycotoxins are found in a staggering range of matrices, from grains to fruits, spices and nuts to animal feed. They are of major concern for their negative impact on the worldwide economy from product revenue losses, their toxicity to animals and humans, and their persistence throughout multiple food processing steps.Learn how Pickering Laboratories addresses this challenge and meets guidelines set by the FDA using sensitive and rugged Thermo Scientific UHPLC methods to effectively detect Aflatoxins as well as other key classes of Mycotoxins. These easy-to-use methods utilize immunoaffinity clean-up and post-column derivatization.

Also join us to discover the benefits of a Thermo Scientific LC-MS/MS method using automated Turbulent Flow sample extraction to separate Mycotoxins from complex food matrices. A highly efficient solution used in high throughput laboratories that eliminates manual sample preparation, improves method reliability and drives down cost per sample.

Who Should Attend
Analytical Chemists
Lab Managers
Food Science Researchers
Presenters
Wendy E. Rasmussen
Wendy E. Rasmussen
Technical Expert
Food, Agricultural and Environmental Markets
Pickering Laboratories
Markus M. Martin
Markus M. Martin
Solutions Manager LC/MS
Thermo Fisher Scientific
Dr. Yang Shi
Dr. Yang Shi
Senior Applications Scientist
LC/MS Applications
Thermo Fisher Scientific
Laura Bush
Moderator:
Laura Bush
Editorial Director
LCGC North America
Presented by
Sponsored by
LC/GC Logo
Thermo Logo
 

AOAC Pacific Northwest Section 2012

Marine and Freshwater Toxins Analysis Third Joint Symposium and AOAC Task Force Meeting
University of Puget Sound, Tacoma WA
June 18-22, 2012
The AOAC Pacific Northwest Section (link: http://www.aoacpacnw.com) annual meeting in Tacoma WA has just concluded. The meeting was full of informative oral presentations and posters ranging from natural disasters to algal blooms due to climate change. One recurring theme is the push to move away from the Mouse Bio-assay (MBA) for shellfish toxin testing. Recently there have been a few AOAC approved methods for toxin testing that do not involve injecting mice. A few oral presentations were updates on the success many labs are having in moving to these chemical methods and reducing the usage of the MBA. One of the successful approved methods is the Post-column Oxidation method for Paralytic Shellfish Toxins (link to PSP method abstract). Both the Pinnacle PCX and the Vector PCX (link to PCX brochure) post-column instruments have been used for this analysis. 
Pickering Laboratories was proud to sponsor the AOAC PNW annual meeting. We presented two posters at this year’s meeting. Please download them from the links and contact support@pickeringlabs.com with any questions.
Method Abstract 103.5: Paralytic Shellfish Toxins
Method Abstract 102: Analysis of Biogenic Amines
Mt. Rainier

Chromatography Quiz #10

Chromatography Quiz #9 Results

We would like to congratulate the grand prize winner of our last newsletter’s Amino Acids Analysis Chromatography Quiz: Jim Balk from the State of Nebraska DHHS Public Health Environmental Laboratory!!!

He has won, and will shortly be receiving: an Amazon.com gift card!  Additionally, all participants will be receiving a gift card from Amazon.com!  Again, we would like to thank all of you for your submissions.  

The correct answer for the modified Amino Acids chromatogram: the sample has been prepared at the wrong pH.  The Aspartic Acid peak is misshapen and splitting, a clear indication that the sample is not sufficiently acidic. After checking the sample pH to determine the required correction, Li220 can be used to adjust to the appropriate pH. 

Thank you! 

Pickering Labs

Chromatography Quiz #10:

Identify the error made when running the Amino Acids chromatogram below and win a prize!  Simply email your answer as well as your full contact information to Rebecca at rlsmith@pickeringlabs.comby August 31st in order to win.  You will receive email confirmation that your submission has been received.  The troubleshooting answer and winner congratulations will be published in the next issue (to be anonymous, please notify Rebecca in submission). 

Hint: Quiz #8 was so popular that we’ve decided to run another hardware problem!

Amino Acid Analysis of Protein Hydrolysate

Pickering Standard: 012506H Protein Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 0.25 µmole/mL, 10 µL injection

Pickering Column: 1193250 Standard Sodium Cation-exchange Column, 3.0 x 250 mm

Normal Operating Conditions: (for reference only, condition changes may be reflected in chromatogram)

Column Temperature: 48 °C

Flow rate: 0.3 mL/min

Eluant Gradient:

TIME
Na328 %
Na740 %
RG0011 %
0
100
0
0
10
100
0
0
32
0
100
0
56
0
100
0
56.1
0
0
100
58
0
0
100
58.1
100
0
0
70
100
0
0

Post-column conditions for amino acid analysis:
Reagent 1: Trione
Reactor 1: 130 °C, 0.5 mL
Reagent flow rate: 0.3 mL/min

Detection: UV-Vis Detector, 570 nm for primary amino acids,  440 nm for secondary amino acids

Chromatogram to Troubleshoot:
Full Chromatogram:

Enlarged View:
Further Enlarged View:

Reference Chromatogram: Click HERE and scroll down to PN 1193250

Pickering Laboratories at Pittcon 2012

The centerpiece of our Pickering Laboratories display at Pittcon 2012 in Orlando, Florida was the FREESTYLE robotic instrument.  Building on the success of the LCTech GPC Ultra sample clean-up instrument,  the completely re-designed FREESTYLE sample clean-up workstation was on view at Pittcon this year. Notable features include an automated liquid handling robot arm, modules for both SPE and GPC, the powerful EVAporation module for online concentration in one package.

 “The FREESTYLE is the culmination of the best of our previous versions and new technology to bring the best automated sample clean-up platform available in the market today “ said Michael Baumann, President of LCTech Germany and manufacturer of the Freestyle.

Laboratory Equipment Magazine was on hand to video tape Mike Gottschalk, VP of Marketing with Pickering Laboratories – highlighting the laboratory productivity and analysis products shown at Pittcon this year:

The Freestyle is a powerful productivity tools for sample clean-up in any high throughput laboratories.

For labs needing Lab-scale sample Clean-up, Pickering Laboratories offers several instruments also on view at Pittcon. The GPC Quattro system enables 4 GPC columns to run simultaneously either all with the same method or with 4 different programs thus providing 4x the productivity of a single column system!

For SPE and Immunoaffinity column processing the EluVac vacuum chamber offers 1 to 20 columns in one setup. With a unique feature that provides these multiple columns to be loaded then washed, and then eluted into the collection vials in one setup just by rotating the cover.  These instruments and columns from Pickering Laboratories provide instruments and consumables for sample clean-up processing. See Wendy Rasmussen demonstrate the EluVac:

Pickering Labs continues to invest in method development for post column-derviatization analysis. The latest new area of focus is the herbal supplement market newly regulated by the FDA. Recently published are methods for Antioxidants in Supplements, Theanine in Tea and Aflatoxin in Spices, coffee and chocolate.
See the complete line of sample clean-up and analysis products on our website: www.pickeringlabs.com

New Mycotoxin Cleanup Columns!

Also on display this year at Pittcon were the a couple of NEW! Mycotoxin clean-up columns available from Pickering Laboratories:
AflaCLEAN SMART
The immunoaffinity column AflaCLEAN SMART is designed for sample clean-up for subsequent Aflatoxin analysis. 
This columns is smaller than the other counterparts on the market – it uses only a fraction of the solvent and yet has 100ng loading capacity as specified by the AOAC methods. 
During extraction, dilution, washing, sample loading and elution, more than 80% of the solvents can be saved.

The final elution volume is only 400uL instead of the typical 2mL – and yet has the same final concentration as with other columns.  As most methods inject only 20-50uL of sample onto the autosampler, this volume is more than enough for analysis. 

These columns can also be used with the very convenient and affordable EluVac vacuum manifold.

If you would like additional information, please email us: sales@pickeringlabs.com

DONeX
The DONeX column can be used in the sample preparation for the analysis of the mycotoxin Deoxynivalenol (Vomitoxin), which is often found on contaminated cereals and grains such as corn. 
The column is ideally suited to many common matrices such as corn, barley, oat, wheat, rye or cereal-based feed. The advantage of DONex is that these columns can also be used with complex matrices and one can stll obtain excellent recovery rates. 
DONeX is used with a sample load of 1 g for HPLC / post column / fluorescence detection or LC / MS.  By increasing the matrix load up to 4 g on column, users with HPLC / UV detection systems can reach detection limits as low as 200 ppb. 
Unlike our other Mycotoxin clean-up columns, DONeX is not an immunoaffinity column, and so it can be stored on the shelf and has an undefined shelf-life.
The DONeX clean-up column is based on a 3mL standard form and thus perfectly suitable for automated handling in the LCTech systems AcceCLEANTM and FREESTLYETM SPE
If you are interested in any additional information or would like to try a sample, please send us an email: sales@pickeringlabs.com

Obsolescence of PCX 5200 in February 2013

Special Note! The Pinnacle PCX and  Vector PCX instruments are available and will continue to be 100% supported for the foreseeable future!

The PCX 5200 will no longer be supported starting February 2013. This means we will no longer keep replacement parts in stock starting February 2013.  

PCX5200 will be obsoleted in February 2013

Common parts like tubing, nuts, ferrules, and filters that are used on the Pinnacle and the Vector will still be available.

Our current models, the Pinnacle PCX and Vector PCX are a great upgrade option to the PCX 5200. You can download our PCX Product Brochure from the Pickering website or find more information by clicking on the individual links.

If you have any questions, please contact support@pickeringlabs.com 

If you would like a quotation for a new system or to place an order, please contact orders@pickeringlabs.com 

David Mazawa,Technical Support Chemist

Chance Favors the Prepared Mind

“Chance Favors the Prepared Mind” – Louis Pasteur
by Michael Pickering 
Chapter One: Preparation
My father and his four brothers were auto/diesel mechanics.  All the families, including grandma and grandpa, moved to Moorpark, CA during World War II. It’s a small agricultural suburb east of Ventura. Small in this context means few paved roads.  The move occurred when I was about two years old.  Probably just after my brother, who was 23 months younger than me, was born.  
They obtained a property on US Highway 101 in Ventura.  The site had three commercial venues: the “Red Ball Café,” a gas station, and a multi-bay garage with a hydraulic lift.  The men managed the auto business and the women saw to the restaurant.  Grandpa was the gofer and I was his shotgun.  Anyone alive today who ate at the Red Ball during that time will have more than one fond memory. 
By the time I was five, properly greasing a wheel bearing and installing it was something I knew how to do by feel, and how to set the cotter pin.  What I learned is that it is extremelyimportant to expel ALL the air pockets before installation.  It didn’t make any sense to me then, but what five year old is going to doubt a team of mechanics.  Moreover, his father and uncles.  Now that I’m the mechanic, I get it: it’s a concept of Chaos Theory.  (See Figure X)
Figure X: A ball bearing from an improperly lubed auto front wheel.  The balls contact the tapered spindle on the inside (attached to the car) and a wheel race pressed into the drum.  All the balls from this wheel looked like this.  The outer race in the drum looked like a choppy sea, yet the spindle was unmarked.  The air bubbles and the ball orientation were maintained in perfect symmetry by chaos. 
Chapter Two: Chance
By the time I was in middle school, we lived in Highland Park, a small town on the first freeway on Earth – the Pasadena.  Freeways then didn’t have numbers; they were named for their termini (the Santa Anna, the San Bernardino, the Harbor, etc.).  We were the penultimate town before the freeway ended at a light signal in Pasadena at Orange Grove Blvd.  
The boulevard through Highland Park is the world famous Figueroa.  Town was about two miles from our neighborhood and a gradual rise most of the way to the movie theater. Since the movies changed once a week, that was our minimum number of journeys.  There were, however, more trips than that in the summer, because the public pool was further up Figueroa.  
Exiting the movies one day, someone, probably me, suggested that we have a coasting contest.  The rules were simple: one could peddle as much as possible to cross street A, about ¼ mile of the course, then not at all to the finish line at cross street B.  Joe McNeil won the first race, and I took second.  
Well, I decided that if I wanted to win, I’d have to beat Joe.  So I began to think of ways to create an advantage.  After all, we all had three speed (hub) bikes, called English racing bikes at the time. (See Figure Y)  When I realized the race was mostly coasting, I also knew the solution was to reduce friction.  Then I remembered hand packing wheel bearings, which is where I started the project.  I removed and replaced all the bearings, including the pedal hub.  Included in the soup-up job was the most expensive waterproof lube I could find.  It felt more like hand lotion than grease.  While I was preparing the bearings, my mind drifted to other ways to lower friction.  It came to me as an epiphany: any wobble of the wheels would cause friction on the bearings and on the road surface.  
Figure Y: A 1953 Schwinn three speed bicycle.
Although my only thoughts about spokes until then had been to keep clothing and body parts out of them, I had changed enough flat tires to know that they were attached to a threaded nut anchored on the OD side of the rim, and the spoke exit on the ID of the rim was a tension adjustment nut.  So it was back to the bike store to buy a new tool.
My first guess was to tune all the spokes to the same audio frequency.  Since I had no way of knowing the true frequency, my second guess was to pick the highest frequency or tightest spoke.  It also simplified the task to require only tightening.  A jig was prepared in the backyard with two 2 x 4’s so the wheels could be levelly mounted and spun.  The spokes were plucked to find the highest frequency, whereupon all spokes were tuned to match.  When done, the rim (as viewed rotating in the jig from a planar angle, perpendicular to the axle) was ‘S’ shaped.  So, Plan B was hatched. 
 
True flatness had to be established by reference to a fixed point.  Another stick with a horizontal nail through it as a palp or feeler was driven into the ground.  The spokes are anchored in turn on opposite sides of the rim.  As the rim turned, it drifted away or toward the feeler gauge.  So the process was: loosen one spoke, and tighten the next.  The eventual result was two true rims. 
Keeping my head down near the handle bar and centered over the quill, to lower friction even more, I never lost another race.